Detection and Phylogenetic Analysis of Enterovirus 71 in Zhenjiang

Objective To detect enterovirus 71(EV71)gene among patients with hand-foot-and-mouth disease(HFMD)in Zhengjiang in 2008 and to analyze the evolution by phylogenetic analysis.Methods The EV71 VP1 genes were amplified by nested PCR.The amplicons were inserted into the vector PET32a(+)and tranducted into the E.coli DH5α.The VP1 gene sequences(891 nucleotides)were determined and compared by phylogenetic analysis.Results The EV 71 VP1 genes were successfully amplified by PCR.The EV71 isolates from Zhenjiang belonged to genotype C by phylogenetic analysis and this genotype was different from the strains isolated from other regions of China.Conclusion The EV71 strains from Zhenjiang belonged to genotype C.