连翘叶片总DNA提取方法的比较

Shanxi Forestry Science and Technology(2007)

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Abstract
以连翘[Forsythia suspensa(Thunb.)Vahl]的新鲜叶片为材料,分别采用CTAB法、高盐低pH法和SDS法3种不同方法提取其总DNA,用琼脂糖凝胶电泳检测获得的DNA的质量,以期从中找出提取连翘总DNA的最适方法。结果表明对于连翘的新鲜叶片,无论从产率上还是质量上,CTAB法都明显优于其他两种方法。
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Key words
Forsythia suspensa(Thunb.) Vahl,DNA extraction,total DNA
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