蓝莓中蛋白硒形态的富集分离及测定方法
Acta Nutrimenta Sinica(2011)
Abstract
目的研究蓝莓中不同形态蛋白硒的富集分离及测定体系。方法非完全硝化—连续提取—巯基棉富集分离,用浓度1.5 mol/L、流速1.0 ml/min的HCl,通过巯基棉富集分离并用2.0 mol/L HNO3加热解脱硒,在优化石墨炉原子吸收法的测定条件下测定。结果丹东地区蓝莓中微量硒含量测定方法的线性回归方程为:A=0.2037C-0.0192(μg/L),可决系数r=0.9832,线性范围0.19~200μg/L;方法检出限(3σ)为12.4 ng/L,回收率为95.6%~106.4%;RSD<4.5%。结论用HNO3-H2O2-HCl的硝化体系、连续提取和巯基棉富集分离,能消除样品中部分干扰离子,使蓝莓中蛋白硒的测定损失少、测定干扰小,结果稳定。
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Key words
blueberry,incomplete nitrification,selenium protein,enrichment separation,GF-AAS
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