Optimization of Seperating 4 Neurotransmitter Amino Acids by Capillary Electrophoresis with Laser-induced Fluorescence

Objective To investigate main influential factors for determination of neurotransmitter amino acids by capillary electrophoresis.Methods 4 neurotransmitter amino acids derivatized withFITC were determined by capillary electrophoresis.Buffer concentrations,pH,surfactants and derivatization conditions were studied and optimized for fast andefficient separation.Results The running buffer concentrations,pH,surfactants and derivatization conditions could be improved for the separating efficiency.4 neurotransmitter amino acids derivatized with FITC were separated on base line in the optimum condition of 75 mmol/L boric acid(pH 9.42)contanining 100 mmol/L SDS acting as running buffer and 50 mmol/L boric acid(pH 9.80) as derivatization buffer in 20 min.Conclusion Through optimization,the separating efficiency of capillary electrophoresis withlaser-induced fluorescence detecting 4 neurotransmitter amino acids can be remarkably improved.