长白山白眉蝮蛇蛇毒酸性PLA_2质谱鉴定及其对血小板聚集的抑制作用
JOURNAL OF BIOLOGY（2010）
Abstract
通过对纯化得到的长白山白眉蝮蛇蛇毒磷脂酶A_2(ABUSV-PLA_2)进行胶内胰蛋白酶酶解,产生的肽段经高效液相色谱-电喷雾串联质谱(HPLC-nESI-MS/MS)进行序列测定,蛋白鉴定采用Sequest Bioworks软件完成.ABUSV-PLA_2与其它蛇毒来源PLA_2的氨基酸序列比对表明:ABUSV-PLA_2是一种新的蛇毒酸性磷脂酶A_2.以ADP诱导的人血小板聚集实验结果表明:ABUSV-PLA_2对ADP诱导的血小板聚集有轻微的解凝效应,但具有显著拮抗血小板的聚集作用,并呈现明显的剂量-效应关系,IC50为356 nmol/L.
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Key words
phospholipase A_2,snake venom,mass spectrometry,platelet aggregation inhibitor
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