Coupling Micropipette Tip-Based Micro-Extraction With Desorption Corona Beam Ionization Mass Spectrometry For Rapid Analysis Of Antihypertensive Drugs In Body Fluid

We developed a convenient method by coupling micropipette tip-based micro-extraction (TBME) with desorption corona beam ionization mass spectrometry (DCBI-MS) for rapid analysis of medicines in biological samples. The operation of TBME is easy and fast, and it can enrich the analyte concentration and eliminate background interference, which makes it suitable as a novel sample preparation method before ambient Ionization. We have systematically optimized the extraction conditions and the instrument parameters. The typical analysis procedure includes the steps of extraction, drying and DCBI-MS detection, and the all processes take less than three minutes. The method was successfully used for the analysis of antihypertensive drugs such as nifedipine, nitrendipine and nimodipine and illegal drugs in body fluid. The method is of considerable interest for broadening the application of ambient ionization mass spectrometry for body fluid analysis.