Transforming Growth Factor-Beta 1 Selectively Recruits Micrornas To The Rna-Induced Silencing Complex And Degrades Cftr Mrna Under Permissive Conditions In Human Bronchial Epithelial Cells

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(2019)

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Abstract
Mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene lead to cystic fibrosis (CF). The most common mutation F508del inhibits folding and processing of CFTR protein. FDA-approved correctors rescue the biosynthetic processing of F508del-CFTR protein, while potentiators improve the rescued CFTR channel function. Transforming growth factor (TGF-beta 1), overexpressed in many CF patients, blocks corrector/potentiator rescue by inhibiting CFTR mRNA in vitro. Increased TGF-beta 1 signaling and acquired CFTR dysfunction are present in other lung diseases. To study the mechanism of TGF-beta 1 repression of CFTR, we used molecular, biochemical, and functional approaches in primary human bronchial epithelial cells from over 50 donors. TGF-beta 1 destabilized CFTR mRNA in cells from lungs with chronic disease, including CF, and impaired F508del-CFTR rescue by new-generation correctors. TGF-beta 1 increased the active pool of selected micro(mi)RNAs validated as CFTR inhibitors, recruiting them to the RNA-induced silencing complex (RISC). Expression of F508del-CFTR globally modulated TGF-beta 1-induced changes in the miRNA landscape, creating a permissive environment required for degradation of F508del-CFTR mRNA. In conclusion, TGF-beta 1 may impede the full benefit of corrector/potentiator therapy in CF patients. Studying miRNA recruitment to RISC under disease-specific conditions may help to better characterize the miRNAs utilized by TGF-beta 1 to destabilize CFTR mRNA.
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Key words
cystic fibrosis, CFTR, TGF-beta 1, microRNA, primary human bronchial epithelial cells
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