The cell lineage arising in response to severe gastric ulceration in the rat is different from the UACL and implicates TFF1 as a differentiation factor

Background: Purinergic stimulation modulates intracellular Ca H [Ca 2 +] j in some cells and has been shown to be important for gastic mucosal healing (Gastro.1995;108:A188).Inhibition of purinergic receptors by suramin inhibits angiogenesis, possibly by direct inhibition of VEGF binding.However, the presence of functional purinergic receptors in rat gastic microvascular endothelial cells (RGMEC) and their role in Ca z + _ signaling and angiogenesis have not been explored.Aim of this study: was to determine the baseline and ATP-driven Ca 2 + signals in RGMEC.Methods: Cultured, subconfluent RGMEC were loaded with 3 /LM Fura-2.Intracellular Ca H was measured by ratiometric imaging of the fluorescence excited by 350-and 385-nm illumination.Results: At baseline,