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产ε-聚赖氨酸白色链霉菌复合诱变选育研究

LI Shuangshuang,WU Zhenqiang,WU Qingping,MO Shuping

China Brewing（2010）

Cited 7|Views35
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Abstract
白色链霉菌(Streptomyces albulus)出发菌株采用紫外诱变和硫酸二乙酯(DES)化学诱变进行复合诱变处理,处理液接种于甘氨酸和多聚赖氨酸抗性平板选育。通过优化甘氨酸和多聚赖氨酸剂量、紫外照射剂量和化学诱变剂DES剂量等诱变条件,并对诱变后的菌株进行初筛和复筛,最后得到一株较高产ε-多聚赖氨酸的菌株,ε-聚赖氨酸的产量达到0.73g/L的较高水平,是出发菌株的2.2倍。经5次传代发酵表明该菌株稳定。
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Key words
ε-polylysine,UV mutation,resistant medium plate,Streptomyces albulus,DES chemical mutation
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