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川芎嗪长循环脂质体包封率的测定

CHEN Daoyang, CHEN Xiaodan, WANG Lisheng

China Medical Herald（2013）

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Abstract
目的 建立川芎嗪长循环脂质体的包封率测定方法.方法 采用葡聚糖凝胶-G50微型柱分离脂质体和游离药物,以高效液相色谱法分别测定含量,并计算包封率.结果 葡聚糖凝胶-G50可使川芎嗪长循环脂质体和游离药物完全分离;HPLC条件下,川芎嗪与辅料和溶剂峰分离良好,川芎嗪浓度在1.09～43.60 μg/mL范围内线性关系良好(r = 0.9994,n = 6).结论 所建立的方法简单可行、重复性好、准确、稳定,为评价和控制川芎嗪长循环脂质体的质量提供了依据.
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Key words
HPLC,Tetramethylpyrazine,Sephadex G-50,Long-circulating liposomes,Entrapment efficiency
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