猴头菌发酵液抑制非酶糖基化反应的培养基优化
Acta Edulis Fungi(2013)
Abstract
以非酶糖基化反应抑制率为评价指标,采用单因素实验筛选影响猴头菌(Hericium erinaceus)发酵液活性的培养基成分,结果表明,①木糖替换PD培养基中葡萄糖,②红豆或绿豆替换PD中马铃薯,③PD添加酵母浸出汁、NaH2PO4·2H2O、维生素B2均可提高抑制率;并在此基础上利用响应面和BoxBehnken Design进一步优化猴头菌液态发酵培养基,当培养基组成为240.50g/L红豆,173.41g/L绿豆,19.49g/L木糖,4.72g/L酵母浸出汁,1.30g/L NaH2PO4·2H2O,56.89mg/L维生素B2,用于猴头920发酵,发酵液对非酶糖基化反应抑制率为(88.51±3.23)%,基本与模型预测值相符,较未优化提高了214%。
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Key words
Hericium erinaceus,optimization of culture medium,non-enzymatic glycosylation(NEG),response surface methodology
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