日本沼虾群体与养殖群体及其杂交后代的同工酶分析
Journal of Aquaculture(2010)
Abstract
采用聚丙烯酰胺凝胶垂直板电泳(PAGE)技术,对日本沼虾野生群体、养殖群体及两者的杂交后代的肌肉组织中乳酸脱氢酶(LDH)、酯酶(EST)、乙醇酸脱氢酶(ADH)和苹果酸脱氢酶(MDH)同工酶进行比较研究。结果表明,日本沼虾不同群体及其杂交后代的LDH、EST和ADH的同工酶可分别稳定地表达出2、3和3条条带,这三种酶表达酶带及表达量差异不显著。MDH同工酶表达2条稳定酶带,在亲本和杂交后代间表达量差异较为显著。
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Key words
isozyme,hybridization,Macrobrachium nipponense
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