Analysis Of Piperine In Peppers Using On-Line Sfe-Uhplc With Photodiode Array Detection

The hyphenated system described in this article couples a supercritical fluid extraction （SFE） system with an ultrahigh-performance liquid chromatography （UHPLC） system. High-speed extraction and the analysis of piperine in pepper powder are demonstrated using an on-line SFE-UHPLC system. The analysis, including the extraction process, was performed in 7.0 min. The extraction recovery was compared with other extraction methods, and the SFE method gave comparable results. The on-line SFE-UHPLC method is very simple and achieves high-speed analysis. The method will be very useful for analyzing the compounds in solid samples without any complicated pretreatment.