siRNA沉默Survivin抑制结肠癌细胞株HCT116增殖并增强其对奥沙利铂的敏感性
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong(2011)
Abstract
目的研究Survivin特异性siRNA对人结肠癌细胞株HCT116增殖和对化疗药物奥沙利铂敏感性的影响。方法体外将Survivin特异性siRNA表达载体pgsiRNA-Survivin转染HCT116细胞,通过Western blot和Real time-PCR方法检测细胞Survivin的表达,采用流式细胞术分析细胞周期分布,MTT法检测干扰Survivin后HCT116细胞对奥沙利铂的敏感性。结果 pgsiRNA-Survivin转染HCT116细胞48 h后,细胞的Survivin基因表达明显降低,导致了HCT116细胞G2/M期阻滞;pgsiRNA-Survivin转染组细胞对奥沙利铂的敏感性显著性增强。结论 Survivin特异性siRNA能显著沉默HCT116细胞Survivin基因,抑制细胞增殖,并增强HCT116细胞对奥沙利铂的敏感性。
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Key words
colon cancer,Survivin,cell proliferation,HCT116 cell,oxaliplatin,RNA interference
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