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Avidin-peroxidase: synthesis and HPLC isolation of highly sensitive oligomers

Gennady V. Gololobov,Alexander M. Shuster,Indulis K. Zalite,Alexander G. Gabibov,Aaron G. Rabinkov

Journal of Biochemical and Biophysical Methods（1990）

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Abstract
Optimal conditions for the preparation of avidin-peroxidase conjugates by the periodate method were studied. A method based on hydrophobic interaction chromatography was developed for the isolation of active obligators. The probable of oligomers with highest sensitivity is discussed.
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Key words
avidin-biotin system,HPLC,glyconjugate
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