超高效液相色谱测定食醋中的六种防腐剂
China Condiment(2013)
Abstract
建立了超高效液相色谱同时测定食醋中6种防腐剂的分析方法。6种化合物经Waters BEHC18(1.7μm,2.1mm×50mm)色谱柱分离,以0.01mol/L乙酸铵(pH 5.0~5.5)和乙腈为流动相进行梯度洗脱,用二极管阵列检测器在波长240nm处进行检测。结果表明:6种防腐剂在8min内可获得良好的分离,加标回收率在93.42%~96.28%之间,相对标准偏差(RSD)在0.75%~1.59%之间。该方法操作简单,灵敏度高,重复性好,可作为检测食醋中防腐剂的方法。
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Key words
preservatives,vinegar,ultra performance liquid chromatography,wavelength detection
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