Effect of particle size and adsorption condition on lipase immobilization in active carbon

The effects of particle size and adsorption condition on lipase immobilized in active carbon were investigated in this paper.The smaller the particle sizes,the higher adsorption and immobilized lipase activity.The stronger lipase adsorption on active carbon might condensate the lipase resulting in lower lipase activity.An increase in lipase concentration from 0.01 to 0.05 mg/mL could improve the activity and absorption of immobilized lipase.The pH and temperature would affect the immobilized activity,but not adsorption.The immobilized lipase activity could reach 65 U/g when prepared by shaking the mixture of 0.05 mg/mL lipase solution at pH 7.0 and active carbon particles of 40 mesh at 150 r/min,30 ℃ for 1 h.