Identification of the suppressive factors for human immunodeficiency virus type-1 replication using the siRNA mini-library directed against host cellular genes.

Masanori Kameoka,Yukiko Kitagawa,Piraporn Utachee,Piyamat Jinnopat,Panadda Dhepakson,Panasda Isarangkura-na-ayuthaya,Kenzo Tokunaga,Hironori Sato,Jun Komano,Naoki Yamamoto,Shinobu Oguchi,Yukikazu Natori,Kazuyoshi Ikuta

Biochemical and Biophysical Research Communications（2007）

Cited 13|Views13

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Abstract

We performed the screening to find the novel host factors affecting human immunodeficiency virus type-1 (HIV-1) replication using the siRNA mini-library consisted with 257 siRNAs directed against cellular genes. J111 cells, a human acute monocytic leukemia cell line, were transfected with individual siRNA, followed by either infected or transfected with the HIV-1 molecular clone with luciferase reporter gene in 96-well plate format. The results showed that six siRNAs significantly enhanced the HIV-1 replication in J111 cells, indicating that the target cellular genes of those siRNAs may negatively regulate HIV-1 replication in normal cell culture condition. We also discuss the possible mechanisms by which those cellular proteins regulate viral replication.

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Key words

Human immunodeficiency virus type-1,RNA interference,Small interfering RNA,siRNA mini-library,Host factor

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