RP-HPLC法测定肠尔舒胶囊中盐酸小檗碱的含量
Northwest Pharmaceutical Journal(2012)
Abstract
目的建立肠尔舒胶囊中盐酸小檗碱含量测定的方法。方法采用RP-HPLC法。色谱柱Sun Fire C18(250mm×4.6mm,5μm);流动相:乙腈-0.05mol·L-1磷酸二氢钾(磷酸调pH为3.0)(25∶75);检查波长:264nm;流速:1.0mL·min-1;柱温:30℃。结果盐酸小檗碱进样量在0.114~1.14μg范围内线性关系良好,r为0.999 9,平均回收率为97.9%(n=9),RSD为1.3%。结论该法准确、简便、快速,可用于肠尔舒胶囊中盐酸小檗碱的含量测定。
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Key words
Changershu Capsules,RP-HPLC,berberine hydrochloride
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