Volume of RBCs, 24- and 48-hour posttransfusion survivals, and the lifespan of 51Cr and biotin-X-N-hydroxysuccinimide (NHS)-labeled autologous baboon RBCs: effect of the anticoagulant and blood pH on 51Cr and biotin-X-NHS elution in vivo

BACKGROUND: The RBC injury that occurs during collection of the first few milliliters of blood Into the pH 5.0 ACD (NIH, Formula A) is referred to as the lesion of collection. The RBC injury was evaluated by labeling the ACD RBCs with Cr-51 and measuring the 24-hour posttransfusion survival. The effect of the acidification of ACD blood on the in vivo elution of Cr-51 from the RBC has not been reported. STUDY DESIGN AND METHODS: Baboon blood was collected In heparin and in ACD and CP2D at different ratios of blood to anticoagulant. ACD blood with a pH of 5.7 to 6.9 was labeled with Cr-51. Heparin[zed blood with a pH of 7.4 was labeled with biotin-X-N-hydroxysuccinimide (NHS). ACD blood with a pH of 5.9 was labeled with both Cr-51 and biotin-X-NHS. The RBC volumes, 24- and 48-hour posttransfusion survivals, and lifespans (T50) were measured. RESULTS: The RBC volume of ACD blood with a pH ranging from 5.7 to 6.9 was not affected by Cr-51 labeling. Cr-51-labeled ACD blood with a pH of 6.9 had an RBC volume that was significantly greater than that seen in heparinized blood with a pH of 7.4 labeled with biotin-X-NHS. In vivo elution of Cr-51 from the RBCs prepared from the ACD blood with a pH of 5.7 to 6.9 and in vivo elution of biotin-X-NHS from RBCs prepared from ACD blood with a pH of 5.9 were associated with reductions in 24- and 48-hour posttransfusion survivals and T50. CONCLUSIONS: The anticoagulant and the pH of the medium in which the RBCs were labeled with Cr-51 or biotin-X-NHS affected in vivo elution of the label from the RBCs and may reduce posttransfusion survival values.