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一例A亚型禽白血病病毒引起的纤维肉瘤的病理学和病毒学分析

Chinese Journal of Animal Infectious Diseases(2011)

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Abstract
将禽白血病A亚型(Avian leukosis virus subgroup A,ALV-A)SDAU09C3毒株人工感染SPF引起的肾脏和肝脏纤维细胞样肉瘤进行病理组织学观察,同时检测是否有其它肿瘤相关病毒感染,另外,应用PCR扩增病毒囊膜蛋白gp85基因,并对其基因编码的氨基酸序列与原攻毒株比较分析.结果显示,SDAU09C3毒株引起的肿瘤,主要为纤维细胞肉瘤,胶原纤维较少,细胞及纤维排列极不规则.病毒学检测证明发病鸡只存在ALV-A的感染,而ALV-J、马立克氏病病毒(Marek's disease virus,MDV)、网状内皮增生症病毒(Reticuloendotheliosis virus,REV)均为阴性.所分离的ALV-A病毒的囊膜蛋白gp85基因编码的氨基酸序列与原攻毒株同源性为99.5%.
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Key words
Avian leucosis virus,fibrosarcoma,A strain
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