冬虫夏草虫草素的提取及提取量的测定
Journal of Dalian Polytechnic University(2013)
Abstract
研究了冬虫夏草虫草素的提取和测定方法,确定了提取冬虫夏草菌株qsun-1菌丝体与甲醇料液比、虫草素的溶剂、超声波细胞破碎的功率和时间。依据单因素和正交试验提出了提取虫草素的最佳工艺:在菌丝体与甲醇料液比(g/mL)为1∶100、超声波功率为300 W条件下,超声波细胞破碎提取30min,虫草素提取量可达9.275mg/g。这些因素对提取虫草素的影响重要性排序为料液比>溶剂>超声波功率>提取时间。717强碱性阴离子交换树脂纯化虫草素提取液,虫草素得率高达80%以上,纯度可达到14.200mg/L。
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Key words
ultrasonic extraction,cordycepin,UV spectrophotometry
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