高效液相色谱法测定蓝莓果浆中糖的组成和含量

Food Science(2009)

Cited 23|Views12
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Abstract
目的:建立高效液相色谱法测定蓝莓果浆中糖的组成和含量的检测方法。方法:超声波水提样品中的糖类,色谱柱为Agilent Zorbax carbohydrate柱(4.6×250mm,5μm),流动相为乙腈-水(75:25,V:V),流速为1ml/min。结果:糖类浓度在0.5~10mg/ml内呈现良好的线性关系,相关系数在0.9989(n=5)以上;精密度实验RSD为0.36%~2.18%(n=5);平均回收率为91.36%~101.34%。结论:该方法简便快速,结果准确可靠,为测定野生蓝莓果浆中糖组分提供了一种高效快速的测定方法。
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Key words
HPLC,glucose,blueberry juice,fructose,sucrose
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