胰腺癌细胞基因组范围内的基因缺失

Lin Lian-Jie,Wang Yu-Feng,Zheng Chang-Qing,Jin Yu,Hu Gang-Zheng,Liu Xiang,Lin Yan

World Chinese Journal of Digestology（2008）

Cited 3|Views4

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Abstract

目的:研究胰腺癌细胞基因组范围内的纯合性缺失和杂合性缺失(loss of heterozygosity, LOH).方法:应用高密度单核苷酸多态性芯片和专用分析软件,检测17种胰腺癌细胞株基因组范围内的纯合性缺失和LOH,并筛选可能与胰腺癌发生、发展有关的基因区域,用PCR验证纯合性缺失.结果:经过PCR验证,26个区域确实为纯合性缺失,芯片的准确度为83.9%(26/31).这些缺失区域中,平均每个区域只涉及1.29个基因.每一种细胞都有不同程度的LOH;不同染色体臂出现LOH频率不同,出现频率最高的为染色体臂9p和18q,均为94.1%.结论:胰腺癌全基因组范围内出现多处LOH和纯合性缺失,这些区域可能含有新抑癌基因.

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Key words

pan- creatic cancer lin lj,hu gz,zheng cq,lin y. genome-wide gene loss in human pancreatic cancer cells.,liu x,homozygous deletion,single nucleotide polymorphism array,jin y,wang yf,loss of heterozygosity

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