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苯酚-硫酸法测定河南丝瓜络多糖的含量

Journal of Xinxiang Medical College(2008)

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Abstract
目的 测定河南产丝瓜络多糖的含量,探讨其药用价值和开发前景.方法 采用苯酚-硫酸法,以葡萄糖(105 ℃干燥至恒重)为对照品测定河南丝瓜络中多糖的含量.结果 在6~91 μg·mL-1的范围内,样品的浓度与吸光度有良好的线性关系,线性回归方程A=8.995 8c- 0.007 8(r=0.999 7),加标回收率101.8%(n=5,相对标准偏差=1.59%),河南丝瓜络多糖的平均含量为7.66%.结论 河南产丝瓜络的多糖含量高于广东、山西和浙江等产地,具有较高的药用价值和开发前景.
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Key words
polysaccharide,retinervus luffac fructus,Henan province,phenol-sulfuric acid method
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