Determination of Seven Metabolites of Magnolia officinalis Rendet Wils.by HPLC

[Objective] To establish an optimal extraction process for simultaneous determination of seven effective metabolites of Magnolia officinalis Rendet Wils.By HPLC method.[Method] The chromatographic conditions were as follows:Samples were performed on the chromatographic column model Shim-pack CLC-ODS(4.6 mm×250 mm,5 m).The mobile phase consisted of acetonitrile(A) and 0.1% formic acid(B).[Result] The regression equation,with peak area(y) as ordinate and concentration(x) as abscissas,of chlorogenic acid,rutin,hyperin,quercitrin,quercetin,magnolol and honokiol,were y =1E+05x-306 360(r=0.999 8),y=5E+04x-79 920(r=0.999 4),y=5E+04x-55 280(r=0.999 9),y=5E+04x-60 530(r=0.999 9),y=2.5E+04x-57 010(r=0.999 8),y=2E+04x+7 102(r=0.999 9) and y=2E+04x+7 102(r=0.999 9),respectively.The concentrations of reference substances showed good linear relationship with peak area within 3.125-100.00 μg/ml.The average recovery rates of chlorogenic acid,rutin,hyperin,quercitrin,quercetin,magnolol and honokiol were 103.98%,101.65%,99.17%,103.59%,103.59%,102.44% and 104.62%,with RSD of 3.37%,2.86%,3.02%,2.89%,2.86%,1.18% and 3.67%,respectively.The content of chlorogenic acid,rutin,hyperin,quercitrin,quercetin,magnolol and honokiol from different parts of Magnolia offiinalis were different from each other.All the 7 effective compounds could be detected in the leaf of Magnolia offiinalis,thereinto,the content of quercitrin,magnolol and honokiol were high,while the content of hyperin and quercetin were low.[Conclusion] The established HPLC method was simple,convenient,stable and with good repeatability,which would provide reference for the further development and utilization of Magnolia officinalis Rendet Wils.