Content Determination of 3 Kinds of Fat-soluble Vitamin in Multivitamin Injection (13) by RP-HPLC

OBJECTIVE:To establish RP-HPLC method for the content determination of vitamin A,vitamin K1,vitamin E in Multivitamin injection (13).METHODS:The determination was performed on Diamonsil C18 column,with mobile phase consisted of methanol at a flow rate of 2.0 mL·min-1 and the detection wavelength was set at 265 nm.RESULTS:The linear ranges were 60～240 μg·mL-1 for vitamin A (r=0.999 7),74～296 μg·mL-1 for vitamin K1 (r=0.999 9) and 4～16 mg·mL-1 for vitamin E (r=0.999 8).The average recoveries were 99.9% (RSD=0.35%),99.9% (RSD=0.32%),100.1% (RSD=0.20%).CONCLUSION:The method is simple,rapid,accurate and reproducible for simultaneous determination of vitamin A,vitamin K1 and vitamin E in Multivitamin injection (13).