Effects of Different Premature Chromosome Condensation Method on Dose-curve of ~(60)Co γ-Ray
Chinese Journal of Radiological Health(2012)
Abstract
Objective To study the effect of traditional method and improved method of the premature chromosome condensation(PCC) on the dose-effect curve of 60Co γ ray,for choosing the rapid and accurate biological dose estimating method for the accident emergency.Methods Collected 3 healthy male cubits venous blood(23 to 28 years old),and irradiated by 0,1.0,5.0,10.0,15.0,20.0 Gy 60Co γ ray(absorbed dose rate:0.635 Gy/min).Observed the relation of dose-effect curve in the 2 incubation time(50 hours and 60 hours) of the traditional method and improved method.Used the dose-effect curve to verify the esposure of 10.0 Gy(absorbed dose rate:0.670 Gy/min).Results ①In the traditional method of 50-hour culture,the PCC cell count in 15.0 Gy and 20.0 Gy was of no statistical significance.But there were statistical significance in the traditional method of 60-hours culture and improved method(50-hour culture and 60-hour culture).Used the last 3 culture methods to make dose curve.②In the above 3 culture methods,the related coefficient between PCC ring and exposure dose was quite close(all of more than 0.996,P0.01),the linear regression equation was established and the correlation index was quite close,the dose-effect curve was fitting the linear model.But there were no statistical significance among regression coefficient(P0.05),the morphology of regression straight lines almost overlap.③Used the above 3 dose-effect curves to estimate the irradiation results(10.0 Gy),the error was less than or equal to 8%,all of them were within the allowable range of the biological experiment(15%).Conclusion The 3 dose-effect curves of the above 3 culture methods can apply to biological dose estimating of large doses of ionizing radiation damage.Especially the improved method of 50-hour culture,it is much faster to estimate and it should be regarded as the first choice in accident emergency.
MoreTranslated text
Key words
Dose Curve Estimating,Accident Emergency,Premature Chromosome Condensation,Methodology Comparison
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined