Involvement of the APC/β-catenin/TCF signaling pathway in the regulatory effects of octreotide on SW480 colon cancer cells

AIM:To investigate whether the APC/β-catenin/TCF signaling pathway is involved in the regulatory effects of octreotide(OCT) on SW480 colon cancer cells.METHODS:(1) mRNA testing:SW480 cells were cultured in vitro and divided into control group and OCT group(treated with 10-10 mol/L OCT).Total RNA was prepared from the two groups of cells and used to detect the mRNA expression of APC2,AXIN,CK1α,cyclin D1 and FZD7 by RT-PCR.(2) Protein testing:SW480 cells were cultured in vitro and divided into control group and OCT group(treated with /L OCT at a concentration of 10-14,10-12 or 10-10 mol/L).Total protein was prepared from the two groups of cells and used to detect the protein expression of APC2,CK1α and CyclinD1 by Western blot.RESULTS:(1) mRNA testing:Treatment with OCT increased the mRNA levels of APC2 and CK1α(both P < 0.05),decreased that of cyclin D1(P < 0.05),but had no significant impact on those of FZD7 and axin(both P > 0.05) in SW480 cells;(2) Protein testing:Treatment with different concentrations of OCT increased the protein levels of APC2 and CK1α and decreased that of cyclin D1 in SW480 cells in a dosedependent manner(all P < 0.05).CONCLUSION:OCT can negatively regulate APC/β-catenin/TCF signaling by up-regulating APC2 and CK1α expression and down-regulating cyclin D1 expression.