Experimental Study of β-elemene Associated with DC/DRibble Vaccine Promoting Spleen Cells Proliferation and Activation

Objective:For elucidating the immune mechanism of β-elemene anti-tumor effect,we studied the effect of β-elemene combined with DC/DRibble vaccine on cell proliferation and IFN-γ secretion level of mice spleen cell in vitro.Methods:Dendritic cells were prepared from Balb/c mice spleen and the phenotype was then identified.Targeting Balb/c mice origin hepatic cancer cell line BNL1MEA.7R.1(BNL),DC/DRibble vaccine was manufacted by recruiting the antigen vector-Drips in blebs(short for DRibble)which are in rich of target tumor cell antigen information.The proliferation effect of β-elemene combined with DC/DRibble vaccine on mice spleen cell was detected by CCK-8 method,the IFN-γ concentration in culture medium was detected by ELISA.Result:In vitro,an appropriate concentration of β-elemene combined with DC vaccine can promote spleen cell proliferation and stimulate the responding lymphocyte to produce high level of IFN-γ.Conclusion:β-elemene may exert its anti-tumor effect via enhancing the DC antigen presenting function.