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氯化汞防治组培苗污染的研究

Northern Horticulture(2012)

Cited 22|Views10
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Abstract
以大花萱草和切花菊组培苗为试材,在MS培养基中分别加入0.005、0.010、0.030、0.050、0.070mg/L氯化汞,研究抑菌剂氯化汞对污染组培苗的消除污染效果。结果表明:当浓度在0.010mg/L时,经过2、3次的转接后,能明显地消除污染。当浓度在0.030mg/L时,1次转接即能对污染起到较好的抑制效果,并且随浓度的增加,抑制效果越好。但浓度超过0.080mg/L时,组培苗迅速出现脱绿死亡现象。综合分析认为,氯化汞的浓度在0.030~0.070mg/L是防治大花萱草和切花菊组培苗污染的最佳浓度。
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Key words
polluted tissue culture seedling,clear pollution,mercuric chloride
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