Preventive Effect of HpaA-VacA IgY on Intragastric Infection with Helicobacter pylori in Mice

Objective To study the preventive effect of HpaA-VacA IgY on the intragastric colonization of Helicobacter pylori and the subsequent inflammation in mice,and lay a foundation of developing IgY-related drugs for both prevention and therapy of H. pylori infection. Methods BALB / c mice were randomly divided into positive control,negative control,infection-preventing and inflammation-preventing groups. The mice in positive control group were administered with 108 cfu / ml H. pylori liquid by intragastric infusion,while those in negative control group with Brucella broth. The mice in infection-preventing group were administered with IgY at various dosages,then with 108 cfu / ml H. pylori liquid. However,the mice in inflammation-preventing group were administered with 108 cfu / ml H. pylori liquid,then with IgY at various dosages. The colonization of H. pylori and severity of inflammatory reaction were observed by culture of intragastric H. pylori and microscopy of stained histological sections. Results The preventionrate of H. pylori infection by HpaA-VacA IgY at a dosage of 6 mg was 88. 9%. However,the HpaA-VacA IgY at a dosage of 8 mgcompletely protected the mice against H. pylori infection and the subsequent inflammation. Conclusion Oral administration with HapA-VacA IgY showed both preventive and curative effects on H. pylori infection in mice,indicating that the IgY might be used for preparation of oral drug for H. pylori infection.