Affinity chromatography of succinate dehydrogenase from the membranes of Micrococcus lysodeikticus.
PREPARATIVE BIOCHEMISTRY(1975)
摘要
Isolated plasma membranes of Micrococcus lysodeikticus were subjected to extraction with n-butanol in a two-phase system. Succinate dehydrogenase obtained in the soluble aqueous phase after high-speed centrifugation was resolved by separation on calcium phosphate gel and affinity chromatography. The affinity ligand used was oxaloacetate and elution from the column was achieved with 0.5 M succinate. In the final product there was an eleven-fold reduction in the 32P-lipid to protein ratio and a fourteen-fold increase in specific activity relative to the high speed supernatant fraction following n-butanol extraction.
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关键词
specific activity,affinity chromatography,calcium phosphate
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