Levels Of Pro-Inflammatory Cytokines And Soluble Cell Adhesion Molecules Serves As Early Markers For Recognition Of Diseases Severity In Patients With Dengue Fever And Dengue Hemorrhagic Fever

Background: Several mechanisms have been proposed to explain the pathogenesis of dengue virus infection. One of the most important finding is the production of proinflammatory cytokines as responsible of activation of vascular wall, plasma leakage and subsequently disease severity. Our aim was to determine levels of pro-inflammatory cytokines during the course and severity of dengue infection. Methods: Seventeen febrile patients with dengue infection (RT-PCR and IgM-anti-dengue) were included and classified by diseases severity (OMS). Dengue fever (DF) were observed in 7/17 cases (2 Adults, 22-56 years old, 39 ± 24.04; and 5 children 3-11 years old, 7.60 ± 3.78); and ten cases had Dengue Hemorrhagic Fever (DHF, 2 Adults; 22-47 years-old, 34 ± 17.67 and 8 children, 5 months-12 year-olds, 4.52 ± 4.27). Two afebrile patients were included as controls. At least two serum samples were collected during either acute phase (<3 days post-infection) and critical phase (4 and more days) of the disease; and stored a −20 °C until used. Serum levels of TNF-&, IL-8, sICAM-1 y sVCAM-1 were determined by commercially available ELISA kits following manufacture instruction. Results: Levels of IL-8 (p > 0.05) and sTNF-& (p = 0.026) were significant higher in DHF cases during the critical phase (≥4d) of the diseases. High soluble levels of ICAM-1 were observed in FD and DHF cases, with a significant differences (p = 0.025) during critical phase of the disease. Moreover, significant soluble levels of VCAM-1 were observed in both cases of DF (p = 0.013) and DHF (p = 0.011) during the early stages of the clinical course (<3d). Conclusion: High levels of TNF-& and IL-8, are correlated with activation of endothelial adhesion molecules sICAM-1 y sVCAM-1 during the critical stages of diseases, especially in DHF where plasma leakage plays a role in vascular damage. Finally, sVCAM-1 expression serves as a early marker for recognition of DHF and SSD. Abstracts for SupplementInternational Journal of Infectious DiseasesVol. 14Preview Full-Text PDF Open Archive