Development Of An In Vivo Radionuclide Generator By Labeling Bleomycin With Os-191

Bleomycin (BLM) has been labeled with various radioisotopes and widely used in therapy and diagnosis. Os-191 is a parent radionuclide with 15.4 day half-life and decays by beta emission to Ir-191m, which is a radionuclide with 4.96 s half-life. BLM was labeled with Os-191-hexachloro-osmate and its distribution and stability in wild-type mice was determined. The complex was obtained at the pH 2 in normal saline at 90 A degrees C in 48 h. Radio-TLC showed an overall radiochemical yield of 93-97%, radio-chemical purity > 97%. The biodistribution study for Os-191-hexachloro-osmate and Os-191-BLM were carried in wild type-mice up to 14 days. Lungs, liver and spleen uptake increased 24-72 h after administration of Os-191-BLM. 24 h after administration, the radioactivity of the kidney increased and remained constant.