实时荧光定量PCR方法检测猪繁殖与呼吸综合征病毒和猪瘟病毒
Chinese Journal of Veterinary Science(2011)
Abstract
根据GenBank发表的猪瘟病毒(CSFV)和猪繁殖与呼吸综合征病毒(PRRSV)的保守区域的序列分别设计了2对特异性引物。建立了一种快速检测CSFV和PRRSV的SYBR GreenⅠ实时荧光定量PCR方法,并用该方法检测采集的临床疑似病料。结果表明,建立的CSFV、PRRSV-SYBR GreenⅠPCR有较好的特异性,敏感性和重复性,可以用于CSFV和PRRSV的检测。
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Key words
classical swine fever,SYBR GreenⅠ real-time PCR,porcine reproductive and respiratory syndrome virus
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