BANNA MINIPIG INBRED LINE (BMI) LIVER-DERIVED CLOTTING FACTORS AND PLASMINOGEN COULD ACTIVATE HUMAN COAGULATION AND FIBRINOLYSIS SYSTEM:

O475* Aims: It had no report about whether porcine liver could maintain normal blood flow after transplanted to human. This study was focus on the possibility and activities of BMI liver derived clotting factors in activating human clotting pathways, BMI plasminogen activated by human tissue plasminogen activator (t-PA) and comparison of antithrombin III between BMI and human. Methods: Prothrombin times (PT) and activated partial thromboplastin time (APTT) tests were conducted by common correction tests with BMI coagulant factors II, V, VII, X and XII and corresponding factor-deficient human plasma. BMI and human plasma plasmin activities tests were conducted by plasmin activities assay kit before and after sera adding human t-PA. The activities of BMI and human plasma antithrombin III were detected with STA-Stago autoanalyzer. Results: BMI clotting factor XII, VII and X could trigger human intrinsic, extrinsic and common coagulation pathway respectively. The activities of BMI clotting factor II, V, VII, X and XII were 3.2, 3.7, 4.7, 2.9 and 4.5 times than those of human. Table 1 BMI and human PT, APTT and clotting factors activityFigureH: human, P: porcine, DHP: deficient human plasma #: Porcine clotting factor activity vs. human clotting factor activity, P<0.01 Table 2 The comparison of plasmin activities activated by human t-PA between BMI and humanFigure#: Statistically significant differences compared with plasma plasmin without human t-PA, P<0.05 * Statistically significant differences compared with human, P<0.05 Table 3 The activities of BMI and human plasma antithrombin IIIFigureConclusions: Our data demonstrated that BMI liver derived II, V, VII, X and XII could trigger human intrinsic, extrinsic and common clotting pathways with much higher activities than those of human. Human t-PA could activate BMI plasminogen into an active plasmin and with higher plasmin activity than that of human. BMI antithrombin III activities were significantly higher than those of human.