Manganese-Dependent Dopa/Tyrosine Sulfation In Hepg2 Human Hepatoma Cells: Novel Dopa/Tyrosine Sulfotransferase Activities Associated With The Human Monoamine-Form Phenol Sulfotransferase

Human monoamine (M)-form phenol sulfotransferase (PST) was PCR-cloned and transiently expressed in COS-7 cells. The recombinant enzyme was demonstrated to display not only the previously reported sulfotransferase activity toward dopamine, but also novel manganese-dependent Dopa/tyrosine sulfotransferase activities. These results imply functional role of the human M-form PST in the homeostatic regulation of Dopa and tyrosine.