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茉莉花愈伤组织诱导及悬浮细胞培养

Journal of Changjiang Vegetables(2011)

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Abstract
以茉莉花(Jasminum sambac)花瓣为外植体在不同培养基上诱导出愈伤组织,通过对其类型的筛选从中选择疏松愈伤组织进行悬浮培养后,初步建立悬浮细胞培养系。结果表明,不同激素可诱导出疏松型(CA)、泥状型(CB)和绿色块状型(CC)愈伤组织,其分化方向也不同。用CA型和CB型愈伤组织均能建立悬浮细胞培养系,其中以泥状愈伤组织为起始材料较佳,大约11 d可建立初步的悬浮细胞培养系。
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Key words
Jasminum sambac,Callus,Cell suspension culture
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