Study on Tissue Distribution of Multiple Effective Components Contained In Qishe Pill in Rats

Objective: To study the tissue distribution of multiple effective components contained in Qishe pill in rats. Method: The heart,liver,lung,kidney,brain and muscle were collected 30 min,1,2,3,4,6 h,after SD rats were given 0. 675 g·kg- 1Qishe pills,then HPLC-MS / MS was used to detect 11 components' concentration in tissues. Result: Flavonoids in Qishe pill was mainly concentrated in brain( calycosin5. 33 μg·g- 1,calycosin-7-glucoside 4. 94 μg·g- 1,formononetin 3. 61 μg·g- 1,ononin 1. 24 μg·g- 1) and lung( calycosin 1.38 μg·g- 1,calycosin-7-glucoside 15.33 μg·g- 1,formononetin 0.29 μg·g- 1,ononin 53.75 μg·g- 1),alkaloids was in muscles( sinomenine 347. 06 μg·g- 1,tetrandrine 706.69 μg·g- 1,fangchinoline 589.07 μg·g- 1),terpenes and organic acids were in brain( senkyunolide A 5. 75 μg·g- 1,senkyunolide I 0. 59 μg·g- 1,ferulic acid2. 4 0 μg·g- 1,cholic acid 56. 82 μg·g- 1). Conclusion: A variety of active ingredients in Qishe pills has a tendency of the lesion site.