c-Jun基因RNA干扰对大鼠血管平滑肌细胞增殖的影响
Chinese Journal of Bases and Clinics in General Surgery(2009)
Abstract
目的以RNA干扰抑制血管平滑肌细胞(vascular smooth muscle cells,VSMCs)的c-Jun基因表达,探讨其表达抑制后VSMCs增殖的变化。方法设对照组(VSMCs不作处理)、阴性si RNA组(VSMCs转染无关序列的si RNA)及c-Jun si RNA组(VSMCs转染c-Jun si RNA)。应用RT-PCR半定量法检测VSMCs中c-Jun的mRNA水平,Western blot法检测VSMCs中c-Jun的蛋白水平,应用MTT比色法及3H-TdR掺入法检测VSMCs的增殖情况,流式细胞仪检测VSMCs的细胞周期变化。结果c-Jun si RNA组的c-Jun mRNA及蛋白表达水平均较对照组明显降低(P<0.05,P<0.01),而阴性si RNA组与对照组间差异无统计学意义(P>0.05)。c-Jun si RNA组VSMCs的增殖活性较对照组明显降低(P<0.05),细胞周期出现明显的G0/G1期阻滞;而阴性si RNA组与对照组间差异无统计学意义(P>0.05)。结论RNA干扰介导的c-Jun基因沉默可显著抑制VSMCs的体外增殖。
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Key words
Activator protein-1,Vascular smooth muscle cell,Intimal hyperplasia,Cell proliferation,RNA interference,c-Jun gene
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