狼尾蕨叶片诱导不定芽植株再生技术
Jiangsu Journal of Agricultural Sciences(2011)
Abstract
以狼尾蕨无菌苗的叶片为外植体,探讨不同类型的细胞分裂素对不定芽诱导、增殖和成苗的影响.结果显示:在附加1.0 mg/L和2.O mg/L KT或1.0 mg/L和2.0 mg/L CPPU的诱导培养基上叶片诱导产生叶状体状不定芽,转到添加0.5 mg/L KT或0.5 mg/L CPPU的增殖培养基上可形成叶芽状不定芽;在附加1.0 mg/L和2.0mg/L BAP的培养基上叶片诱导可产生叶状体状不定芽,转到添加0.5 mg/L BAP的增殖培养基上不定芽仍保持叶状体状.叶芽状不定芽在附加0.5 mg/L CPPU的增殖培养基上连续继代培养,每代芽增殖系数达5以上.叶芽状不定芽转到附加0.2 mg/L IBA的成苗和生根培养基上,能发育成具有根、茎、叶的完整小植株,成苗率达100%.
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Key words
adventitious bud,cytokinin,Davallia bullata.,plant regeneration,leaf
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