鳄鱼血SDS-PAGE鉴别方法的研究

Chinese Journal of Pharmaceutical Analysis(2011)

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Abstract
目的:建立鳄鱼血SDS-PAGE鉴别方法。方法:利用电泳缓冲液制备鳄鱼血、绵羊血、豚鼠血、鸡血、兔血、鹿血等6种动物全血样品液,采用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法,对6种动物全血进行鉴别研究。结果:以7.5%~11%的梯度分离胶、60μg蛋白量上样,大板(16 cm×20 cm)进行SDS-PAGE鉴别,6种动物全血的电泳谱带有明显差异,鳄鱼血存在特征条带。结论:本方法灵敏度高、专属性强,可作为鳄鱼血与其他动物血的鉴别依据。
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Key words
crocodile blood,antineoplastic,electrophoresis,antimicrobial drug,characteristic bands,antivirotic,SDS-PAGE
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