Sciatic Nerve Regeneration Using A Nerve Growth Factor-Containing Fibrin Glue Membrane(Star Star Star Star)
Neural regeneration research(2013)
Abstract
Our previous findings confirmed that the nerve growth factor-containing fibrin glue membrane provides a good microenvironment for peripheral nerve regeneration; however, the precise mechanism remains unclear. p75 neurotrophin receptor (p75(NTR)) plays an important role in the regulation of peripheral nerve regeneration. We hypothesized that a nerve growth factor-containing fibrin glue membrane can promote neural regeneration by up-regulating p75(NTR) expression. In this study, we used a silicon nerve conduit to bridge a 15 mm-long sciatic nerve defect and injected a mixture of nerve growth factor and fibrin glue at the anastomotic site of the nerve conduit and the sciatic nerve. Through RT-PCR and western blot analysis, nerve growth factor-containing fibrin glue membrane significantly increased p75(NTR) mRNA and protein expression in the Schwann cells at the anastomotic site, in particular at 8 weeks after injection of the nerve growth factor/fibrin glue mixture. These results indicate that nerve growth factor-containing fibrin glue membrane can promote peripheral nerve regeneration by up-regulating p75(NTR) expression in Schwann cells.
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Key words
neural regeneration,nerve growth factor-containing fibrin glue membrane,p75 neurotrophin receptor,Schwann cells,peripheral nerve regeneration,fibrin glue,nerve growth factor,peripheral nerve injury,sciatic nerve,microenvironment,grants-supported paper,neuroregeneration
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