On cDNA cloning and expression analysis of GRAS gene in fingered citron

A fragment of EST was isolated from fingered citron by suppression subtractive hybridization.It was cloned the full-length cDNA of the EST by RACE.The result showed that this fragment was highly associated with GRAS genes of Arabidopsis and the sequence alignment showed that it had a conserved domain of GRAS.Therefore,the GRAS gene of fingered citron could be named as CmsGRAS(GenBank accession number: JF440647),which had an ORF of 1 236 bp and encoded 413 amino acids.Fluorescence quantitative PCR method was used to study the expression characteristics of GRAS gene in fingered citron under low temperature stress,the results showed that the gene expression significantly changed after injured by low temperature.