Breeding of Industrial Diploid Yeast Strain with Chromosomal Integration of Multiple β-Glucosidase Genes

Satoshi Saitoh,Tsutomu Tanaka,Akihiko Kondo

Journal of Bioscience and Bioengineering（2008）

引用 20|浏览2

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摘要

We constructed a double auxotrophic OC-2 industrial diploid strain of Saccharomyces cerevisiae and introduced 4 copies of cell surface displaying β-glucosidase (BGL) genes into the chromosome. The engineered OC-2 strain showed 5-fold higher BGL activity compared with the yeast carrying 2 copies of BGL gene and directly produced ethanol from cellobiose.

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关键词

yeast,cellulosic ethanol,β-glucosidase,chromosomal integration,fermentation

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