Experimental Study of Canine Bone Marrow Stromal Cells Cultured by Autologous Serum in vitro

Objective:The purpose of this study was to investigate whether cell culture medium of autologus serum is more effective on expansion and differentiation of canine bone marrow stromal cells(BMSCs).Methods:One adult beagle dog was used in this study.10%and 20%autologous serum medium were prepared.BMSCs were obtained from bone marrow of the canine.Experiments were divided into three groups:A,BMSCs cultured in 10%autologus serum;B,BMSCs cultured in 20%autologus serum;C,BMSCs cultured in 10%fetal bovine serum(FBS).From the second generation of cells,β-glycerophosphate,ascorbic acid and dexamethaso were added to the three culture medium for osteogenic induction.The alkaline phosphatase and calcium nodules were examined by immunohistochemistry.The morphology of BMSCs,cells counting and MTT assay were detected.Quantitative data were expressed as means±SD,SPSS software version 13.0 for Windows was used.Results:Morphology of the BMSCs in A,B and C groups were similar.Calcium nodules could be observed in all groups after induction.There were no significant differences between groups in statistical analysis.Conclusion:Preparation of autologous serum can completely replace FBS for culture of bone marrow stromal cells.