[Aberrant expression of sperm protein 17 enhances migration of ovarian cancer cell line HO-8910].
Zhonghua nan ke xue = National journal of andrology(2008)
摘要
OBJECTIVE:To explore the effects of aberrant expression of sperm protein 17 (Sp17) on the migration of the ovarian cancer cell line HO-8910.
METHODS:The recombinant plasmid pEGFP-Sp17 containing Sp17 and enhanced green fluorescent protein gene was transfected into the human ovarian cancer cell line HO-8910 with Lipofectamine 2000. The expression of Sp17 was examined by RT-PCR and Western blot, and the cell migratory capability detected by Transwell chamber assays.
RESULTS:Sp17 was expressed as a fusion protein with EGFP after transfected. There was a significant difference in the migratory cell number of the transfected and the control cells (156.6 +/- 14.9/HP vs 39.3 +/- 8.53/HP, P < 0.05).
CONCLUSION:The aberrant expression of Sp17 greatly enhances the migration of ovarian cancer cells.
更多查看译文
关键词
migration,ovarian cancer,sperm protein 17
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要