Applicability of phylogenetic methods for characterizing the public health significance of verocytotoxin-producing Escherichia coli strains.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY(2008)
摘要
Two phylogenetic methods (multilocus sequence typing [MLST] and a multiplex PCR) were investigated to determine whether phylogenetic classification of verocytotoxin-producing Escherichia coli serotypes correlates with their classification into groups (seropathotypes A to E) based on their relative incidence in human disease and on their association with outbreaks and serious complications. MLST was able to separate 96% of seropathotype D and E serotypes from those that cause serious disease (seropathotypes A to Q, whereas the multiplex PCR lacked this level of seropathotype discrimination.
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关键词
phylogeny,multiplex pcr,virulence,sequence alignment,escherichia coli,polymerase chain reaction,public health,multilocus sequence typing,cluster analysis
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