大肠杆菌RNaseⅢ基因的克隆与表达
Letters in Biotechnology(2008)
Abstract
目的:克隆并在原核表达系统中表达RNaseⅢ基因。方法:提取大肠杆菌JM109株的基因组DNA,以之为模板扩增得到RNaseⅢ基因全序列,将该编码序列克隆到原核表达载体pET-22b(+)中,转化大肠杆菌BL21(DE3),IPTG诱导重组RNaseⅢ表达。结果与结论:在大肠杆菌中克隆到了RNaseⅢ的全基因,经测序证明与数据库中报道的序列一致;表达的重组RNaseⅢ主要以包涵体形式存在。
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Key words
RNaseⅢ,prokaryotic expression,gene cloning
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